KMID : 0861020190340020075
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Korea Journal of Herbology 2019 Volume.34 No. 2 p.75 ~ p.82
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Effect of Curcuma longa L. Extract on the Melanin Accumulation and Expression of Skin Fibril Proteins
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Kim Hae-Ok
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Abstract
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Objectives : In this study, various biological effects of Curcuma longa L. have been studied, however, beneficial effect of Curcuma longa L. in skin health remain still unclear. In this study, Curcuma longa L. water extract (CLE) was prepared. Inhibitory effect of CLE on melanin accumulation of B16F10 cells and expression levels of skin fibril-related proteins of human skin fibroblasts (HSF) were evaluated.
Methods : The cytotoxic effect of CLE in B16F10 cells and HSF were examined by MTT assay. Inhibitory effect of CLE on the ¥á-MSH- and IBMX-induced melanin accumulation and tyrosinase activity were evaluated in B16F10 cells. The expression levels of connective tissue growth factor (CCN2), Smad2, procollagen 1¥á2, collagen 1¥á2, and fibronectin in CLE-treated HSF were analyzed by western blotting.
Results : The CLE treatment (concentrations 10 to 400 ¥ìg/ml) for 72 h did not affect to the B16F10 viability. However, 200 and 400 ¥ìg/ml of CLE treatment for 24 h showed cytotoxic effect in HSF. Therefore, the concentrations 10, 50, and 100 ¥ìg/ml of CLE were chosen in this study. The CLE treatment for 72 h dose dependently and significantly suppressed melanin accumulation and tyrosinase activity of B16F10 cells. In addition, the CLE treatment up-regulated expression levels of skin fibril-related proteins such as CCN2, Smad2, procollagen 1¥á2, collagen 1¥á2, and fibronectin.
Conclusions : In conclusion, these results suggest that the CLE could be used as a natural material for skin health.
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KEYWORD
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Curcuma longa L., melanin, tyrosinase, skin, skin fibril
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